DEC-2005-IHC Envision+ method

<tab class=wikitable sep=bar head=top border=1> Headline1|Headline2|Headline3 Aceton cold 50%|In ventilation|30 s Aceton cold 100%|In ventilation |5 min Wash|TBS|2 x 5 min Primary antibody |E-cadherin in humidity chamber
1/9000 diluted in TBS |60 min Wash|TBS + TWEEN 20
TBS: 500ml of the stock
(6.06 g TRIS/HCl from Merck L479219-136 + 1.39 g TRIS/base from Sigma
T1503 in 1ldH2O check pH7.6) in 5l NaCl 0.87% (87g NaCl from Merck K33224404-418 in 10 l dH2O)|2 x 5 min Inactivation of peroxidase|peroxidase block from the EnVision+ kit|5 min Wash|TBS|2 x 5 min Secondary antibody|EnVision+ mouse
(DAKO)|30 min Wash |TBS ± TWEEN 20|2 x 5 min Visualisation|DAB
20 ul + 1 ml buffer
(1 drop DAB+ 1 ml buffer)|5 min Wash|Running water|10 min Counterstain|Hematoxylin|4 min Wash|Running water |10 min Alchool|70 %|30 s Alchool|96 %|2 min Alchool|Absolut|2 min Toluen, xylol|In ventilation|5 min Mounting of sections|Eukit|20 min </tab>