View source for DEC-2005-IHC Envision+ method

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'''Headline1'''|'''Headline2'''|'''Headline3'''
Aceton cold 50%|In ventilation|30 s
Aceton cold 100%|In ventilation |5 min
Wash|TBS|2 x 5  min 
Primary antibody |E-cadherin  in humidity chamber<br>1/9000 diluted  in TBS |60 min
Wash|TBS + TWEEN 20<br>TBS: 500ml of the stock<br>(6.06 g TRIS/HCl from Merck L479219-136 + 1.39 g TRIS/base from Sigma<br>T1503 in 1ldH2O check pH7.6) in 5l NaCl 0.87% (87g NaCl from Merck K33224404-418 in 10 l dH2O)|2 x 5 min
Inactivation of peroxidase|peroxidase block from the EnVision+ kit|5 min
Wash|TBS|2 x 5 min
Secondary antibody|EnVision+ mouse<br>(DAKO)|30 min
Wash |TBS &plusmn; TWEEN 20|2 x 5 min
Visualisation|DAB<br>20 ul + 1 ml buffer<br>(1 drop DAB+ 1 ml buffer)|5 min
Wash|Running water|10 min
Counterstain|Hematoxylin|4 min
Wash|Running water |10 min
Alchool|70 %|30 s
Alchool|96 %|2  min
Alchool|Absolut|2 min
Toluen, xylol|In ventilation|5 min
Mounting of sections|Eukit|20 min
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[[Category:Protocols]]
[[Category: Daniela Elena Costea]]
[[Category: Oral Pathology Dept.]]
[[Category: Haukeland Hospital]]
[[Category: Norway]]